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How to Clean a Liquid Nitrogen Dewar or Tank: Proper Sanitization and Decontamination

Proper maintenance and cleaning of your liquid nitrogen LN2 dewar or tank is crucial to ensure its longevity and the safety of your stored materials. In this blog, we’ll guide you through the essential steps to sanitize, decontaminate, and clean your LN2 dewar, so you can maintain its performance and prevent corrosion.

Many users underestimate the importance of cleaning the inside of their liquid nitrogen tanks. During regular use, moisture accumulates inside the container, and it can become contaminated with unwanted substances like bacteria or even biological materials. These contaminants can cause corrosion and negatively impact the contents of your tank. For instance, if semen accidentally mixes with the liquid nitrogen, it can lead to corrosion and a decrease in the conception rate of artificial insemination.

emptying an ln2 dewar
  1. Prepare the Dewar:
    • Start by removing the cap, all liquid nitrogen and any other contents from the container. Allow the dewar to sit for about two days, allowing the internal temperature to rise.
  2. Wash with Warm Water or Neutral Detergent:
    • Once the container has warmed up, inject warm water at a temperature between +40°C and +50°C into the liquid nitrogen tank. You can also use a neutral detergent for this step. Gently scrub the interior with a cloth to remove any contaminants.
  3. Rinse Thoroughly:
    • After scrubbing, rinse the tank with clean water to remove any remaining residues.
  4. Dry the Dewar:
    • Invert the container to allow it to dry completely. You can choose between natural air drying or hot air drying but ensure the drying temperature does not exceed +50°C. Avoid temperatures above +60°C, as they may affect the dewar’s performance and lifespan. Air circulation will help speed up the process.

When you are selecting a cleaning solution, it’s essential to consider the materials your dewar is made from. For example, the inner vessel of an MVE aluminum cryogenic dewar is constructed with aluminum and utilizes a fiberglass neck support. You can use a household detergent or mild soap solution, as these cleaners are safe for aluminum and stainless-steel components.

To thoroughly sanitize your liquid nitrogen dewar, you can use a 10% chlorine bleach with a 90% water solution. Ensure that all inner surfaces of the dewar are swathed with this solution for at least 30 minutes. Then, rinse the decontaminated surfaces with clean water and remove any rinse water. Allow the dewar to dry completely before putting it back into service. Inverting the dewar to drain and dry is highly recommended.

a clean liquid nitrogen dewar

For the exterior of the dewar, a light dampened cloth with a mild soap solution can be used to clean any stains, contaminants, or condensation. Make sure to completely dry the exterior once you are finished cleaning. Regular cleaning of the exterior of the dewar will prevent corrosion and maintain the bottom of the vessel.

Proper cleaning and maintenance of your liquid nitrogen LN2 dewar are essential to keep it in optimal condition and ensure the safety of your stored materials. Follow the steps outlined in this guide and choose the right cleaning solutions to keep your dewar in top shape. By following these practices, you can prevent corrosion, maintain the conception rate of artificial insemination, and ensure the long-lasting performance of your LN2 dewar.